lecture10 - Effects of Cholesterol on Membranes: Physical...

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Effects of Cholesterol on Membranes: Physical Properties Removes gel to liquid crystal phase transition New “intermediate” phase called liquid ordered - ordering of the membrane lipids due to condensation (closer packing, less trans-gauche isomerizations) Cholesterol “thickens” bilayers with 16 C or less Cholesterol “thins” bilayers with 18 C or more Modulation of physical properties and minimizing permeability to small molecules
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Suppression of phase transition to LC state NMR, EPR, fluorescence, DSC
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FRAP : Flourescence Recovery After Photobleaching Used to determine viscosity: relevant to effects of cholesterol, lateral phase separation and RAFTS
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Permeability : Hypothesis: gauche-Trans isomerizations lead to small gaps or holes of “free volume ” space. The idea is that the constant movement of the bilayer will make a series of connected holes to allow small molecules (smaller than glucose) to pass. Cholesterol reduces the number of gauche-trans isomerizations by ordering the hydrocarbons, thus limiting the permeability.
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This note was uploaded on 09/19/2011 for the course CHM 6304 taught by Professor Richards during the Fall '08 term at University of Florida.

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lecture10 - Effects of Cholesterol on Membranes: Physical...

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