lecture13 - lytic, transmembrane domain comprises the lower...

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~ 30 - 40% of the genes of an organism code for membrane proteins ~ 70% of current pharmaceuticals target membrane proteins ~ 110 structures of membrane proteins; whereas over 30,000 structures of globular proteins in the RSCB Protein Data Bank Pancreas cell
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Topography Mapping: Proteolytic Cleavage Chemical Labeling Sequence Prediction Accessibility Measurements
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Membrane Protein Topography beta-barrel alpha helical Stephen White website: UC Irvine http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html
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Abstract The structure of the Staphylococcus aureus alpha-hemolysin pore has been determined to 1.9 A resolution. Contained within the mushroom-shaped homo-oligomeric heptamer is a solvent-filled channel, 100 A in length, that runs along the sevenfold axis and ranges from 14 A to 46 A in diameter. The
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Unformatted text preview: lytic, transmembrane domain comprises the lower half of a 14-strand antiparallel beta barrel, to which each protomer contributes two beta strands, each 65 A long. The interior of the beta barrel is primarily hydrophilic, and the exterior has a hydrophobic belt 28 A wide. The structure proves the heptameric subunit stoichiometry of the alpha-hemolysin oligomer, shows that a glycine-rich and solvent-exposed region of a water-soluble protein can self-assemble to form a transmembrane pore of defined structure, and provides insight into the principles of membrane interaction and transport activity of beta barrel pore-forming toxins. TolC...
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lecture13 - lytic, transmembrane domain comprises the lower...

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