9-25-08 - E4P Trp Tryptophan Tryptophan ATP: Histidine O -O...

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Trp E4P
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Tryptophan Tryptophan ATP: Histidine
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- O O O H - O O O CH 3 - O O O H 2 C COO - - O O O H 2 C H 2 C COO - - O O H H NH 3 + glutamate alpha-KG glutamate alpha-KG glutamate alpha-KG - O O H CH 3 NH 3 + Glycine Alanine - O O H H 2 C COO - NH 3 + Aspartate NH 3 NADPH, ATP NADP+, ADP + P i - O O H H 2 C H 2 C COO - NH 3 + Glutamate NH 3 ATP ADP + P i NH 3 ATP AMP + PP i - O O H H 2 C NH 3 + O NH 2 - O O H H 2 C H 2 C NH 3 + O NH 2 glutamine asparagine glyoxalate pyruvate oxaloacetate alpha-ketoglutarate
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α KG + NADPH + NH 3 + ATP ---> glutamate + NADP + + ADP + P i Remember the cost of glutamate Making glycine, alanine, aspartate, glutamate costs 1 NADPH and 1 ATP Making glutamine costs an additional ATP Making asparagine costs an additional 2ATP
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COO - C N H 2 + CH 2 H 2 C H 2 C H COO - C NH + CH 2 H 2 C HC H + H 3 N C COO - CH 2 H CH 2 C O H + H 3 N C COO - CH 2 H CH 2 C O - O Proline Glutamate- γ-σεμ ιαλδεηψδε δεηψδρογ ενασε Γ λυταμ ατε κινασε ανδ Γ λυταμ ατε-γ-σεμ ιαλδεηψδε δεηψδρογ ενασε Νον-ενζψμ ατιχ Νον-ενζψμ ατιχ Προλινε οξιδασε 1 -Πψρρολινε-5-χαρβοξψλατε ρεδυχτασε 1 Γ λυταμ ατε
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α KG + 3NADPH + NH 3 + 2ATP ---> proline + 3NADP + + 2ADP + 2P i α KG + NADPH + NH 3 + ATP ---> glutamate + NADP + + ADP + P i
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Proline degradation Proline Pyrroline-5-carboxylate H + N C COO - H N N NH N R O O N C O NH 2 R H H FAD NADPH N N NH N - R O O H O O N N NH N R O O H H A O H Reactive Flavin H H H B H A N N NH N R O O H HO N H + C COO - H B H A N N NH N R O O
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N H + C COO - H H O H B H A N H 2 + C COO - H H A O H B H C COO - NH 3 + CH 2 CH 2 C O H Glutamate semialdehyde Pyrroline-5-carboxylate Non-enzymatic
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H C COO - NH 3 + CH 2 CH 2 C O H E S H B H C COO - NH 3 + CH 2 CH 2 C - H E S N + C NH 2 O R H C COO - NH 3 + CH 2 CH 2 C O E S N C NH 2 O R NADP + NADPH H C COO - NH 3 + CH 2 CH 2 C O E S H O - B H A H C COO - NH 3 + CH 2 CH 2 C O O - Glutamate semialdehyde dehydrogenase Glutamate semialdehyde Glutamate
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So….Proline = glutamate If: Glu = α -ketoglutarate + NADPH + NH 3 Then: Pro = α -ketoglutarate + NADPH + NH 3 If: α -ketoglutarate = 4 NADH + 2 GTP + 2 FADH 2 + NADPH Then: Pro = 4 NADH +2 GTP + 2 FADH 2 + 2 NADPH + NH 3 If: NADH = 3 ATP GTP = ATP FADH 2 = 2 ATP NH 3 = -2 ATP Then: Pro = 16 ATP + 2 NADPH
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COO - C + H 3 N CH 2 H HN C COO - CH 2 H CH 2 C O H HN C COO - CH 2 H CH 2 C O - O Arginine N-acetylglutamate- γ-σεμ ιαλδεηψδε δεηψδρογ ενασε Ν-αχετψλγ λυταμ ατε κινασε ανδ Ν-αχετψλγ λυταμ ατε-γ-σεμ ιαλδεηψδε δεηψδρογ ενασε Ν-αχετψλγ λυταμ ατε-γ- σεμ ιαλδεηψδε Γ λυταμ ατε ΧΗ 2 ΧΗ 2 Η Ν Χ + Η 3 Ν Χ ΧΟΟ - ΧΗ 2 Η ΧΗ 2 Χ Ο - Ο Χ Ο Η 3 Χ Ν-αχετψλγ λυταμ ατε Αχετψλγ λυταμ ατε σψντηασε Χ Ο Η 3 Χ ΗΝ Χ ΧΟΟ - ΧΗ 2 Η ΧΗ 2 ΧΗ 2 + Η 3 Ν Χ Ο Η 3 Χ Ν-αχετψλορνιτηινε ΝΗ 2 + Η 2 Ν + Η 3 Ν Χ ΧΟΟ - ΧΗ 2 Η ΧΗ 2 ΧΗ 2 + Η 3 Ν Ορνιτηινε Ν-αχετψλορνιτηινασε αμ ινοτρανσφερασε
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9-25-08 - E4P Trp Tryptophan Tryptophan ATP: Histidine O -O...

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