10-02-08 - COO- B COOH C H CH2 CH2 CH2 CH2 COO- CH2 NH SB...

Info iconThis preview shows pages 1–15. Sign up to view the full content.

View Full Document Right Arrow Icon
H C COO - NH 3 + CH 2 CH 2 CH 2 CH 2 NH COO - CH 2 CH 2 C COO - O H B H C COO - NH 3 + CH 2 CH 2 CH 2 CH 2 N COO - CH 2 CH 2 C COO - SB H- H+ H C COO - NH 3 + CH 2 CH 2 CH 2 CH N COO - CH 2 CH 2 CH COO - H H B NAD+ H C COO - NH 3 + CH 2 CH 2 CH 2 CH N COO - CH 2 CH 2 CH COO - SB H C COO - NH 3 + CH 2 CH 2 CH 2 CH H 2 N COO - CH 2 CH 2 CH COO - O Lysine Degradation NADPH
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
N C H 2 N O R H C COO - NH 3 + CH 2 CH 2 CH 2 C H O N + C H 2 N O R E H B H C COO - NH 3 + CH 2 CH 2 CH 2 C S O - H E H C COO - NH 3 + CH 2 CH 2 CH 2 C S O E H C COO - NH 3 + CH 2 CH 2 CH 2 C S O E - O H B H A H C COO - NH 3 + CH 2 CH 2 CH 2 C O - O Transamination C COO - CH 2 CH 2 CH 2 C O - O O α -ketoadipate NAD+
Background image of page 2
O C COO - CH 2 CH 2 CH 2 C O O - O C CH 2 CH 2 CH 2 C O O - TPP dependent oxidative decarboxylation S CoA glutaryl-CoA alpha-ketoadipate O C CH CH 2 C O O - S CoA Fatty acid beta oxidation C H 2 - O C CH CH 2 S CoA C H H+ O CH CH 3 S CoA C H FADH2 NADH
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
O CH CH 3 S CoA C H FA beta oxidation O CH 2 CH 3 S CoA C H OH O CH 2 CH 3 S CoA C O FA beta oxidation 2 H 3 C O S CoA NADH
Background image of page 4
So…Lysine = NADH + α -ketoadipate + 2 NH 3 If: α -ketoadipate = 2 NADH + FADH 2 + 2 Acetyl-CoA Then: Lys = 3 NADH + FADH 2 + 2 Acetyl-CoA + 2 NH 3 If: Acetyl-CoA = 3 NADH + FADH 2 + GTP Then: Lys = 9 NADH + 3 FADH 2 + 2 GTP + 2 NH 3 If GTP = ATP, NADH = 3 ATP FADH 2 = 2 ATP and NH 3 = -2 ATP Then: Lys = 35 ATP
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
oxaloacetate + 3NADPH + NH 3 + 3ATP ---> threonine + 3NADP + + 3ADP + 3P i
Background image of page 6
COO - H NH 3 + H 2 C H 2 C OPO 3 2- COO - H NH + H 2 C H 2 C OPO 3 2- N H + B H+ COO - NH + H C H 2 C OPO 3 2- N H + H B - NH + C H CH 2 N H + OH- H+ - NH + C H 3 N H + OH H B H+ - NH + C H 3 N H + H gamma lyase/beta synthase
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Threonine can be degraded in three ways (1) Threonine dehydrogenase pathway Threonine Dehydrogenase H C + H 3 N COO - O H CH 3 H B NAD+ H C + H 3 N COO - O CH 3
Background image of page 8
H C COO - NH 3 + C OH S CH 3 CoA Must break C α -C β bond on an amino acid and stabilize a carbanion
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
H C COO - NH 3 + C OH S CH 3 CoA N H + 2- O 3 PO O - C H O Schiff Base Formation N H + 2- O 3 PO O - C H H C COO - NH + C O S CH 3 CoA H B N H 2- O 3 PO O - C H H C COO - NH + H 3 C C O S CoA H A N H + 2- O 3 PO O - C H H C COO - NH + H Schiff Base Hydrolysis H C COO - NH 3 + C O CH 3 H S CoA B A N H + 2- O 3 PO O - C H O COO - C H H NH 3 + Glycine α-αμ ινο-β-κετοβυτψρατε 2-amino- α -ketobutyrate lyase
Background image of page 10
What happens to the glycine? Glycine Cleavage System: trifunctional enzyme - OOC CH 2 NH 3 + N + 2- O 3 PO O - H C H O Schiff Base Formation N + 2- O 3 PO O - H C H C C NH + H H O O - CO 2 N 2- O 3 PO O - H C H C NH + H H S S E N + 2- O 3 PO O - H C H C NH + H H S E S - H A STEP1: decarboxylation of glycine
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
N + 2- O 3 PO O - H C H O Schiff Base Hydrolysis N + 2- O 3 PO O - H C H C NH + H H S E SH N H N N R H H S E - CH 2 NH 2 B N H N N R H CH 2 H 2 N HS E HS B H A N H N N R CH 2 NH 3 N 5 ,N 10 -methylene tetrahydrofolate Tetrahydrofolate H A THF N 5 ,N 10 -methylene-THF STEP 2: formation of N 5 ,N 10 -methylene-THF
Background image of page 12
S H H N + C O NH 2 R B E S S N C O NH 2 R NAD + NADH STEP 3: regeneration of catalytic site
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
There is another pathway of glycine degradation From glycine cleavage system Combine the glycine cleavage system with a reverse of the
Background image of page 14
Image of page 15
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 53

10-02-08 - COO- B COOH C H CH2 CH2 CH2 CH2 COO- CH2 NH SB...

This preview shows document pages 1 - 15. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online