Adv RNA Splicing Lecture I&II.Swanson Spr2006

Adv RNA Splicing Lecture I&II.Swanson Spr2006 -...

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adcnfjvancacedurrkilnnbudamascspe wlicaing Reviews General: Curr. Opin. Cell Biol. 13: 302 (2001) Nat. Rev. Mol. Cell. Biol. 4:960 (2003) Alternative splicing: Ann. Rev. Biochem. 72:291 (2003) Splicing and disease: Genes Dev. 17:419 (2003) Mol. Cell. Biol. 24:10505 (2004) Research article: Cell 120:59 (2005) Maurice Swanson R1-118G mswanson@ufl. Advanced RNA Splicing
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Finding Splice Sites in a Sea of Intronic Sequence The giant elastic protein titin is encoded by a single gene located in human and mouse on chromosome 2. The human titin gene is composed of 363 exons that code for a total of 38,138 amino acid residues (mol. mass 4200 kDa). re cellular transcripts spliced?How complicated can it be?
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The Two-Step Splice of Life
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Produce a labeled RNA transcript by in vitro transcription Pre-mRNA Splicing Mechanism: In Vitro Biochemical Analysis Incubate in HeLa nuclear extract with ATP
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Pre-mRNA Splicing Mechanism: In Vivo Analysis In vivo assays Transfection analysis Philips et al, Science 280:737 (1998)
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Some Clues About Splice Sites Splice donor consensus -4 -3 -2 -1 +1 +2 +3 +4 +5 +6 5’ C 29 /A 34 C 38 /A 35 A 62 G 77 G 100
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This note was uploaded on 09/20/2011 for the course BCH 4024 taught by Professor Allison during the Fall '08 term at University of Florida.

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Adv RNA Splicing Lecture I&II.Swanson Spr2006 -...

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