BCH4024-Technology

BCH4024-Technology - Recombinant DNA Technology Reading:...

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Recombinant DNA Technology The discovery that DNA was the genetic material, followed by work which solved its structure and provided a means for how it could be replicated, began the modern era of molecular biology. However for molecular biology to grow to what it has become today, required the development of many techniques for handling DNA, since it is a very large and complicated molecule. BCH4024 Dr. Robertson, R3-226 392-1810, keithr@ufl.edu
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Figure 25B.1 Creation of recombinant DNA molecules in vitro. [page 971] Recombinant DNA – joining 2 different DNA molecules together Restriction enzyme – endonuclease, usually isolated from bacteria, that cuts at a defined DNA sequence
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Figure 25B.2 Cloning a fragment of DNA into a plasmid vector and introducing the recombinant molecule into bacteria. [page 971] Cloning – creating many identical copies of a DNA sequence for analysis ‘Transformation’ Select for bacteria that took up plasmids by plating on media with antibiotic
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Figure 25B.4 pBR322, one of the earliest plasmid cloning vectors. [page 972]
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BCH4024-Technology - Recombinant DNA Technology Reading:...

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