Chapter 8

Chapter 8 - MCB 2000 - Exam 2 Notes Chapter8...

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MCB 2000 - Exam 2 Notes Chapter 8 Microbial Metabolism 0 Metabolism •Collection of ALL biochemical reactions that take place within cells of an organism •The ultimate function of metabolism is to reproduce the organism Anabolism •Building of complex molecules from simpler ones •Process by which a cell is built up •Energy requiring process (endergonic) •Consumption of energy Energy + A + B   (enzyme) C •Catabolism •Process by which complex molecules are broken down into simpler ones  - energy released (exergonic) 0 Release of energy X + Y (enzyme)   Z + Energy  Metabolism includes: •Nutrient uptake  Conversion of nutrients into cell components •Conversion of nutrients into energy •Excretion if waste products •Catabolism: Getting Materials and Energy -Glucose is the most frequent nutrient used for obtaining energy There are three metabolic pathways in which glucose is transformed: Glycolysis ( splitting of glucose) -Occurs in cytoplasm of most cells -Involves splitting of a 6-carbon glucose into two 3-carbon sugar molecules 0 -Direct transfer of phosphate between two substrates -   substrate level phosphorylation 0 High energy P directly transferred from a substrate to  ADP 1 C-C-C~P + ADP -> C-C-C + ATP 1 Net gain of (products of glycolysis):
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MCB 2000 - Exam 2 Notes 0 Two ATP molecules 1 2 molecule of NADH 2 Pyruvic acid 1 •3-carbon molecules are oxidized to  0 -2 molecules of pyruvic acid 1 -2 NAD 2 NADH+ 2 -4 ATP formed 2 •Energy balance of Glycolysis = 2 ATP Aerobic Respiration:  Krebs Cycle (Tricarboxylic Acid Cycle) •A series of biochemical reactions in which chemical energy released step by step •Starting compound is pyruvic acid (from glycolysis) that is transformed into Acetyl CoA •Acetyl CoA is decarbonized (loss of CO 2  molecule) and entered into Tricarboxylic acid  cycle Anaerobic respiration ATP generating process (O 2  not present) -Final electron acceptor is an inorganic molecule
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Chapter 8 - MCB 2000 - Exam 2 Notes Chapter8...

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