Chapter 17

Chapter 17 - Chapter17: 1) Directtesting(microscopic, Timerequired:fromfewminutes Timerequired:fewdaystofewweeks( Basedonsymptoms(athletesfoot)

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 17: Diagnosing Infections  1) How to Identify the Disease  -Direct testing (microscopic, immunological genetic methods)  -Time required: from few minutes…  -Cultivation of microorganisms  -Time required: few days to few weeks (mycobacterium tuberculosis)  -Based on symptoms (athlete’s foot)  2) How to Identify Unknown Bacteria  -Phenotypic Methods  -Macroscopic morphology  -Microscopic Morphology  -Physiological/ Biochemical Characteristics  -Genotypic Methods  -Culturing not necessary  -Results obtained quickly  -Immunological Methods  -Looking for the specific antibody in patient’s blood  3) Specimen Collection  -Can be collected by a member of a clinical team or b a patient  -Aseptic procedures should be followed 
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
-Avoid taking samples of normal biota (e.g. saliva when taking throat swab *can  lead to misleading results and conclusions*)  -Samples should be promptly transported to the lab  4) Immediate Direct Examination of Specimen  -Direct microscopic observation  -Fresh  -Stained (Gram staining, acid fast staining)  -Direct fluorescence antibody test  -Antibody labeled with a fluorescent dye  5) Cultivation of Specimen  -A dominant organism has to be isolated into a pure culture  -The isolated microorganism is characterized by  -Microscopic morphology  -Staining reaction (G+ or G-)  -Cultural appearance  -Motility  -Oxygen requirements  -Biochemical characteristics  6) Biochemical Tests  -Enzymatic activity (fermentation of glucose, lactose…, production [email protected] presence of catalase, use of citric acid as sole carbon source. ..) 
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/16/2011 for the course MCB 2000 taught by Professor Gantar during the Fall '08 term at FIU.

Page1 / 8

Chapter 17 - Chapter17: 1) Directtesting(microscopic, Timerequired:fromfewminutes Timerequired:fewdaystofewweeks( Basedonsymptoms(athletesfoot)

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online