Micro Notes - Chapter Three (1)

Micro Notes - Chapter Three (1) - Chapter3 Inoculation...

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Chapter 3 Inoculation -Introducing a sample (the inoculum) into a container with a nutrient medium -The medium contains appropriate nutrients that sustains the growth of microorganisms -Some microbes have to be inoculated into a living organism Isolation: Separating One Species from Another -Obtaining a pure culture -Cultures composed of cells arising from a single cell -> PURE CULTURE Obtaining a Pure Culture 1) Streak Plate Method 2) Dilution Method Types of Media 1) Physical State a. Liquid b. Solid (agar) 2) Chemical Composition a. Synthetic b. Non-Synthetic (complex) 3) Functional Type a. General purpose b. Enrichment c. Selective d. Differential -Measures taken when working with microbiological media -Needs to be sterilized -Prevents contamination Synthetic Media -Known chemical composition Ex. – NaN – 3gl glucose Non-Synthetic Media -Contains chemically undefined components Ex. – Pepton, Beef extract
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Enrichment Media -Supports the growth of a specific group of microorganisms Ex. – N2-fixing Selective Media -Favor specific microorganisms and inhibits the others (Methylene blue inhibits the growth of Gram+ bacteria) Differential Media -Contains substances that permit detection of microorganisms with specific  metabolic activity (Blood agar) Incubation -Microbiological cultures are placed in temperature-controlled chambers –  incubators -Temperature 20-40 degrees Celsius  Microscope – The Instrument -Microscopes are the instruments that magnify the cell (object) to extent at which  the cell details become visible
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This note was uploaded on 09/16/2011 for the course MCB 2000 taught by Professor Gantar during the Fall '08 term at FIU.

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Micro Notes - Chapter Three (1) - Chapter3 Inoculation...

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