SIMPLE ENZYME REACTIONS 521 methyl-red. Run in M 10 HCl from the first burette A 1 until a red colour is obtained identical with that in B. Note the difference in the volume of acid run in from A 1 and B 1 . Calculation. The equations for the above reactions are: CO(NH 2 ), + 2H 2 O = (NHJ 1 CO, (NHJ 2 CO 8 + 2HCl = 2NH 4 Cl + CO 1 + H 2 O i gram-molecule of urea = 2000 ml. of A/.HCl solution i.e., 60 g. of urea = 2000 ml. of A/.HCl solution .*. 0-003 g- of urea = i ml. of A//IO HCl solution Aldehyde oxidase (or dehydrogenase). This enzyme, sometimes also called the Schardinger enzyme, occurs in milk. It is capable of "oxidising" acetaldehyde to acetic acid, and also the purine bases xanthine and hypoxanthine to uric acid. The former reaction is not a simple direct oxidation and is assumed to take place as follows. The enzyme ** activates " the hydrated form of the aldehyde so that it readily parts with two hydrogen atoms in the presence of a suitable hydrogen acceptor such as methylene-blue
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This note was uploaded on 09/19/2011 for the course CHM 2211 taught by Professor Castalleano during the Fall '06 term at University of Florida.