Lab 5 Report - 1 John Malloy BMZ115EK Tzvia Cuperman...

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1 John Malloy BMZ115EK – Tzvia Cuperman October 18, 2010
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2 Hypothesis: If Aspergillis flavus and Aspergillis niger are grown together, the growth of both species will be hindered, but the A. flavus will out-compete the A. niger. Methods: The hypothesis, “If Aspergillis flavus and Aspergillis niger are grown together, the growth of both species will be hindered, but the A. flavus will out-compete the A. niger, ” was tested using three potato dextrose agar plates. A culture of A. niger and a culture of A. flavus were each grown in their own agar plate. One agar plate was then given a sample of both species. The materials we used included: three agar plates, pure cultures of A. niger and A. flavus , acetate sheets with .5 cm squares to determine growth, and 2 cork borers. The very first step taken, was the collection of all the required materials. After that, a cork borer was used to place a sample of A. niger in the middle of one of the agar plates. Immediately after that, A. niger was placed slightly off-center in the second agar plate, which will end up holding both species. Next, a sample of A. flavus was placed in the center of the third plate. The final step was to place A. flavus slightly off-centered in the second plate. The three plates appeared as follows: plate one has a sample of A. niger in the center, plate two has a sample of both species slightly off-centered, and plate three has a sample of A. flavus in the center. After all the cultures were plated, they were covered and left out to grow for five days, at which point they were placed into a refrigerator to stop the growth. This experiment had several controlled variables. The pH of all the plates was unaltered, meaning that every plate had a 5.5 pH. The other controls related to how the plates were left out. The exact temperature that they were stored at was relatively unimportant (as long as it was close to room
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Lab 5 Report - 1 John Malloy BMZ115EK Tzvia Cuperman...

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