exam1 09 key - NAME_KEY MIDTERM EXAM I BIOLOGY 231 17...

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NAME: __________ KEY _______________ MIDTERM EXAM I BIOLOGY 231 17 September 2009 ________________________________________________________________________________ -1- GENERAL INSTRUCTIONS: 1. Answer all questions IN INK. 2. NO CALCULATORS. 3. Confine each answer to the BOX or SPACE provided. You are free to use the backs of the pages for scratch work, but ONLY what is written in the box or space will be graded! 4. SHOW YOUR WORK in all calculations. 5. Make sure that your name is PRINTED LEGIBLY on EACH PAGE. 6. This exam consists of 7 questions on 16 pages and it is worth a total of 100 points. Information that may be useful: < For the reaction A + B <==> C + D, Ä G = Ä G + RTln([C][D]/[A][B]) o and RTln(X) = 5.7 kJ/mol @ log(X) eq < At equilibrium, Ä G = 0 and Ä G = ! 5.7kJ/mol A log(K ) o < log (10 ) = X; 10 = X X log(X) < surface of sphere = 4 ð r ; volume of sphere = 4/3 ð r ; area of circle = ð r ; surface of cube = 6e 23 2 2 < 1M = 10 mM = 10 ì M; 1 mole = 10 mmoles = 10 ì moles 36 3 6 < For an enzyme that obeys Michaelis-Menten kinetics, the relationship between V and [S] is: MAX V = V × OR, [S] M ([S] + K ) = + × M 1 1 K 1 V V V [S] < An amino acid has the following structure: < On this date in 1787, the US Constitution was signed in Philadelphia by 38 of the 41 delegates present. It is now the longest-standing, longest-observed written constitution in the world.
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NAME: __________ KEY _______________ MIDTERM EXAM I BIOLOGY 231 17 September 2009 ________________________________________________________________________________ -2- R groups of some amino acids, shown in their charged form, if relevant: ala (A): ser (S): glu (E): gly (G): lys (K): arg (R): val (V): tyr (Y): his (H): trp (W): leu (L): asp (D):
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NAME: __________ KEY _______________ MIDTERM EXAM I BIOLOGY 231 17 September 2009 ________________________________________________________________________________ -3- pK values of some amino acids amino acid pK of side chain or terminal group alanine (ala or A) uncharged arginine (arg or R) 12.5 asparagine (asn or N) aspartic acid (asp or D) 3.9 Cysteine (cys or C) 8.3 glutamic acid (glu or E) 4.3 glycine (gly or G) histidine (his or H) 6.0 leucine (leu or L) lysine (lys or K) 10.8 Phenylalanine (phe or F) serine (ser or S) tryptophan (trp or W) tyrosine (tyr or Y) 10.9 Valine (val or V) peptide N terminus 7.8 peptide C terminus 3.6
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NAME: __________ KEY _______________ MIDTERM EXAM I BIOLOGY 231 17 September 2009 ________________________________________________________________________________ -4- (1/10) x 10,000 ì m = 10 ì m 33 3 (1) The drawings below show a cell in which a lipid bilayer is represented as a single line. The cell in (1) contains an endosome with a trans-membrane protein represented as a “lollipop” and a free soluble molecule “X,” both orientated as shown. Imagine that a fission or budding event splits the endosome into two separate vesicles: a large one, “A” that retains both the membrane protein and the soluble molecule, and a smaller vesicle, “B,” that contains neither. This is shown in drawing 2. Next, the vesicle “A” undergoes exocytosis: it fuses with the plasma membrane, while vesicle B remains in the cytoplasm, as shown in (3). (a) 2 pts. In the drawing 2, DRAW in BOTH the transmembrane protein and the soluble molecule X as they would be orientated in organelle A. (b) 2 pts. In the drawing 3, DRAW in BOTH the transmembrane protein and the soluble molecule X in their proper location and orientation. (c) 2 pts. Assume that the organelle in drawing 3 is spherical. If the volume of the cell is 10,000 ì m and the radius of the organelle is 1/10 the radius of the cell, what is 3 the volume of the organelle? Answer in the box below.
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exam1 09 key - NAME_KEY MIDTERM EXAM I BIOLOGY 231 17...

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