25 27 Oct 2010

25 27 Oct 2010 - LEHNINGER PRINCIPLES OF BIOCHEMISTRY Fifth...

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Unformatted text preview: LEHNINGER PRINCIPLES OF BIOCHEMISTRY Fifth Edition David L. Nelson and Michael M. Cox 2008 W. H. Freeman and Company CHAPTER 9 DNA-Based Information Technologies Paul Berg was awarded the 1980 Nobel in Chemistry "for his fundamental studies of the biochemistry of nucleic acids, with particular regard to recombinant-DNA" and shared with Gilbert and Sanger for DNA sequencing methods Herbert Boyer and Stanley Cohen combined their efforts in biotechnology to invent a method of cloning genetically engineered molecules in foreign cells recombinant human insulin produced by bacteria Cloning of DNA from any organism entails five general procedures 1. Cutting DNA at precise locations. Sequence specific endonucleases (restriction endonucleases) provide the necessary molecular scissors. 2. Selecting a small molecule of DNA capable of self-replication. These DNAs are called cloning vectors (a vector is a delivery agent). They are typically plasmids or viral DNAs. 3. Joining two DNA fragments covalently. The enzyme DNA ligase links the cloning vector and DNA to be cloned. Composite DNA molecules comprising covalently linked segments from two or more sources are called recombinant DNAs....
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25 27 Oct 2010 - LEHNINGER PRINCIPLES OF BIOCHEMISTRY Fifth...

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