Lecture 5, protein trafficking, with movie

Lecture 5, protein trafficking, with movie - Membrane...

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Membrane Traffic: The Secretory Pathway The RER (rough endoplasmic reticulum), Signal Peptides, Membrane Proteins
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Eukaryotic cells are highly compartmentalized, with many membrane compartments
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Membrane Traffic ** Secretory traffic, starting at endoplasmic reticulum vs. Endocytic traffic
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The Endoplasmic Reticulum (ER) is a dynamic structure: visualized with Green Fluorescent Protein (GFP) fused to a protein that resides in the ER
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Electron micrograph of some membranes in secretory pathway “free” ribosomes Golgi stacks Cooper 9.22 The ER membrane is an extension of the outer nuclear membrane. With ribosomes associated with it ˰ rough ER (RER). RER (rough ER)
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Figure 12-41a Molecular Biology of the Cell (© Garland Science 2008)
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Free vs. bound ribosomes in eukaryotes How do “bound” ribosomes bind the RER? or inserted into ER membrane Cooper 9.3 In some eukaryotes (e.g., baker’s yeast), proteins enter ER mostly by post-translational translocation. Post-translational Co-translational translocation
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Proteins that are targeted to different cellular compartments often have specific signal sequences Table 12-3 Molecular Biology of the Cell (© Garland Science 2008)
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Cooper 9.6 ER signal sequence of growth hormone (a secreted protein): targeting signal to the RER Most ER signal sequences for a secreted protein: an amino-terminal (N-terminal) stretch of nonpolar (hydrophobic) residues, often flanked by more positively charged residues (i.e. arginine in this case) on one side than the other.
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The Nobel Prize in Physiology or Medicine 1999 was awarded to Günter Blobel "for the discovery that proteins have intrinsic signals that govern their transport and localization in the cell".
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S ignal R ecognition P article (SRP) Composed of 6 distinct polypeptides + Small RNA molecule (7SL) SRP Binds ER Signal Peptide
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This note was uploaded on 09/19/2011 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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Lecture 5, protein trafficking, with movie - Membrane...

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