Lecture 6, coated vesicles

Lecture 6, coated vesicles - The Secretory Pathway:...

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The Secretory Pathway: Principles of vesicular traffic
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After the ER, proteins are transported to the Golgi Figure 13-3a Molecular Biology of the Cell (© Garland Science 2008)
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Golgi: distinct areas Figure 13-25a Molecular Biology of the Cell (© Garland Science 2008)
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N-linked oligosaccharides are MODIFIED in the Golgi (DON’T MEMORIZE) N-linked carbohydrates are trimmed and then additional carbohydrates are added. Figure 13-31 Molecular Biology of the Cell (© Garland Science 2008) Congenital disorder of glycosylation (CDG syndromes): relatively rare birth defects that lead to serious malfunction of different organs.
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Figure 13-32 Molecular Biology of the Cell (© Garland Science 2008) Some proteins are O-linked glycosylated in the Golgi (or serine) N-acetylgalactosame added first, followed by additional sugar residues in the Golgi
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What are some of the known functions of protein glycosylation? 1. Assists in protein folding. 2. Restricts access to surface of glycosylated protein (e.g., making such protein more resistant to protease). 3. Part of feature recognized by other molecules (e.g., proteins) on cell surface (e.g., during cell-cell adhesion).
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trans-Golgi network (TGN) (acid phosphatase staining) cis-Golgi stacks (osmium staining) medial-Golgi stacks (nucleoside diphosphatase staining) Enzymes reside in distinct stacks of the Golgi Figure 13-27 Molecular Biology of the Cell (© Garland Science 2008) No special staining
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Figure 13-28 Molecular Biology of the Cell (© Garland Science 2008) In addition to their biological effects on proteins, these modifications serve as markers of where a protein (e.g., Bip) has been (like stamps in a passport)
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Figure 13-3b Molecular Biology of the Cell (© Garland Science 2008)
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Three basic players : Donor membrane, target membrane, and transport vesicle Figure 13-2 Molecular Biology of the Cell (© Garland Science 2008) (e.g., ER) (e.g., cis Golgi)
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Vesicle budding accomplished by COATS that surround vesicles COATS: peripheral membrane proteins function as: a mechanical devise that can bend and curve a vesicle, (vesicle budding) a mechanism for selecting the cargo proteins to be included in the vesicle Small GTPase regulates the coating process
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Lecture 6, coated vesicles - The Secretory Pathway:...

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