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Unformatted text preview: chromosomes. RNA processing None Introns, 5’ cap, 3’ Poly A Post-transcriptional regulation miRNA, siRNA Cytoplasmic polyadenylation (unique), miRNA, siRNA mRNA Unstable, decays in few minutes Stable and can last hours, days or months or a lifetime. Sigma factors Needs sigma factors (70 and 54) to help RNA Polymerase bind No sigma factors. RNA polymerase subunits has similar functions. Translation Same time as transcription Separate processes. mRNA must be exported before translation can occur Ribosomes 70S, 3 different rRNA molecules 80S, 4 different rRNA molecules 1) Tatabox binding protein binds to Tatabox. 2) TFIIB binds to DNA and TBP to secure both. 3) TFIIF binds to RNA Polymerase II and both bind to DNA just downstream of Tatabox as TBP recognizes RNA polymerase. 4) TFIIE binds. 5) TFIIH binds. Its subunit, kinase phosphorylates the CTD, promoting elongation. 6) After initiation, everything dissociated except TBP....
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