BIBC 102 Homework 2 11

BIBC 102 Homework 2 11 - Metabolic Biochemistry Summer 2011...

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Metabolic Biochemistry Name_____________ Summer 2011 Homework 2 (40 pts.) Section____________ 1) (8 pts.) The following data was collected from kinetic analysis performed on new enzyme: 5 22 [S] ( µ mol/L) V 0 ( µ mol/L)/min 10 39 20 65 50 102 100 120 200 135 a. Prepare a Lineweaver-Burke plot from the data and estimate V max and K M (show your work) . You can do this either on graph paper or using a graphing program on a computer (MS Excel works fine). You must turn in either your plot on graph paper or a printout. b. If the total enzyme concentration is 1 nmol/L, and enough substrate has been added to achieve the maximum velocity for the reaction (V max ), how many molecules of substrate are converted to product by one molecule of enzyme in one second? (Show your work.) 2) (8 pts.) Sketch a Michaelis_Menten plot (V 0 vs. [S]—not a reciprocal plot) for an enzyme with and without a competitive inhibitor. Clearly label V max and K M with and without the inhibitor.
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3) (8 pts.) From the textbook, chapter 13, answer question 15 on page 524.
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This note was uploaded on 09/20/2011 for the course BIBC 102 taught by Professor Price during the Summer '02 term at UCSD.

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BIBC 102 Homework 2 11 - Metabolic Biochemistry Summer 2011...

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