BIBC 102 HW 2

BIBC 102 HW 2 - Metabolic Biochemistry Summer 2010 Homework...

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Metabolic Biochemistry Name_____________ Summer 2010 Homework 2 (40 pts.) Section____________ 1) (8 pts.) The following data was collected from kinetic analysis performed on new enzyme: [S] ( mol/L) V 0 ( mol/L)/min 5 22 10 39 20 65 50 102 100 120 200 135 a. On graph paper, prepare a Lineweaver-Burke plot from the data and estimate V max and K M. b. If the total enzyme concentration is 1 nmol/L, and enough substrate has been added to achieve the maximum velocity for the reaction (V max ), how many molecules of substrate are converted to product by one molecule of enzyme in one second? (Show your work.) 2) (8 pts.) Sketch a Michaelis_Menten plot (V 0 vs. [S]—not a reciprocal plot) for an enzyme with and without an irreversible inhibitor. You can assume that the enzyme molecule is destroyed when it reacts with the inhibitor. Clearly label V max and K M with and without the inhibitor.
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3) (8 pts.) In glycolysis, glucose-6-phosphate is converted to fructose-6-phosphate. If the reactants and products have the following concentrations at equilibrium
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BIBC 102 HW 2 - Metabolic Biochemistry Summer 2010 Homework...

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