section 2 handout_answers

section 2 handout_answers - Bio1A Spring 2011 Discussion...

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Bio1A Spring 2011 Discussion Section Handout #2 Week of January 31, 2011 Terms to Know Light microscope Organelle Scanning electron microscope Transmission electron microscope Cell fractionation Plasma membrane Cytosol Chromosomes Ribosomes (free and bound) Prokaryotic cell Eukaryotic cell Nucleus Endoplasmic reticulum (smooth and rough) Endomembrane system Golgi apparatus Lysosome Vacuole Vesicle Mitochondria Chloroplast Peroxisome Cytoskeleton Microtubules Microfilaments Intermediate filaments Centrosome/centriole Cilia Flagella Myosin Actin Cell wall Plasmodesmata Extracellular matrix Tight junctions Desmosomes Gap junctions Selective permeability Amphipathic Fluid mosaic model Freeze-fracture Peripheral proteins Integral proteins Transmembrane proteins Diffusion Osmosis Tonicity Isotonic solution Hypertonic solution Hypotonic solution Transport proteins Aquaporins Facilitated diffusion Channel protein Carrier protein Active transport Membrane potential Electrochemical gradient Electrogenic pump Exocytosis Endocytosis *Plasma membrane *Cytosol *Chromosomes *Ribosomes *Bacteria and Archaea *No nucleus *Highly compacted DNA in an unbound region called the nucleoid *No membrane-bound organelles *Cytoplasm bound by the plasma membrane *1-5μm diameter *Many have a cell wall Eukaryotic Cells Prokaryotic Cells *Protists, fungi, animals, and plants *DNA in a nucleus bound by a nuclear envelope *Membrane-bound organelles *Cytoplasm in the region between the plasma membrane and the nucleus *10-100μm diameter
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1. What are some advantages and disadvantages of both light and electron microscopy? What are the limits on magnification and resolution for each type of microscope? LM EM Advantages: Can image living cells About 100x better resolution than LM Can see color Can see subcellular structures Can prepare samples without much processing Disadvantages: Resolution not as good as EM Can’t see living cells Magnification not as good Can’t see color Samples must be prepared in a destructive way/preparation may introduce artifacts
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This note was uploaded on 09/20/2011 for the course CHEMISTRY 120 taught by Professor Head-gordon during the Spring '11 term at ASU.

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section 2 handout_answers - Bio1A Spring 2011 Discussion...

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