hw6 - BIO 5099 Homework Assignment #6

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Unformatted text preview: BIO 5099 Homework Assignment #6 Name_____________________________________ Due start of class, Tuesday October 1st. Late assignments are not accepted, show your work for partial credit. Assignments may be e-mailed to: christiaan@xiaan.com or rbaror@dmibio.com anytime, or faxed to (303) 556-2889 between 3:00 and 4:00 p.m. on Tuesday. Define in your own words and give an example: 1. (2pts) Hydrolysis 2. (2pts) Substrate Affinity 3. (2pts) Isoelectric Point 4. (2pts) Antibody 5. (2pts) How does a ribosome know where to start translation? Where to stop translation? 6. (2pts) Why? Which is more efficient: aerobic metabolism or anaerobic metabolism? 7. (8pts) Vitamin C deficiency is a contributing factor for scurvy. a) What enzyme is affected by the Vitamin C deficiency? b) What is this enzyme's EC Number? What are the two top level EC classifications for this enzyme? b) How does Vitamin C interact with this enzyme? c) What does this mean for collagen produced as a result of a diet deficient in Vitamin C? 8. (2pts) For Michaelis-Menten Kinetics, what are the assumptions of the steadystate approximation? What does this mean for [E] vs. [S]? 9. (2pts) How do we measure an enzyme's affinity for substrate? If an enzyme has a Km of 14.5, what is its affinity? 10. (2pts) In the space below, draw the reaction curve for a typical enzyme, plotting [S] versus the reaction velocity. In the same space, show how this curve would be affected in the presence of a competitive inhibitor to this enzyme. V Vmax [S] 11. (2pts) Describe two differences between light and electron microscopy. You are a scientist in a large laboratory with every imaginable instrument at your disposal you even know how to use them all! You have been assigned to characterize an unknown sample of either DNA or protein, handed to you in a small vial. 12. (5pts) Describe what instruments/techniques you would use to determine whether the sample contains DNA or protein (consulting other scientists doesn't count). 13. (4pts) Assume the sample contains protein. What instruments/techniques would you use to characterize/identify it? 14. (3pts) Assume the sample contains DNA. What instruments/techniques would you use to characterize/identify it? ...
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This note was uploaded on 09/20/2011 for the course CSC BIO 5099 taught by Professor Prof.larryhunter during the Fall '02 term at University of Colorado Denver.

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