Fall 2002 Midterm Answers

Fall 2002 Midterm Answers - Name _ Student number _...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Name _____________________________ Student number ____________________ University of Guelph Department of Chemistry and Biochemistry Chem*2580 Introductory Biochemistry Midterm Examination. October 22, 2002. Time allowed, 75 min. Answer questions 1-20 on the computer scoring sheet provided. Only one option is correct for each of these questions. If your computer scoring sheet has space for a nine digit student number, leave two spaces on the left blank and enter the number in the seven spaces on the right (see figure). Use pencil to fill in the circles representing your chosen answer; and erase cleanly if you make an error. Do not use ink or white-out on the computer scoring sheet. You may mark your answer on the question paper for your own records, however in case of a discrepancy between question paper and the computer scoring sheet, the choice shown on the computer sheet will be taken as final. Answer questions 21-24 directly on the question paper. Constants and other data required for numerical problems can be found on the last page. 40 marks total ======= 20 marks Questions 21-24 have marks as indicated 20 marks Questions 1-20 have a weight of 1 mark each
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Answer the questions 1-20 on the computer scoring sheet. Value 1 mark each 1. The catalytic triad of chymotrypsin consists of the amino acids B) Asp, His, Ser ___________ 2. Which of the following amino acids causes ammonia to be released when it is hydrolysed by acid? C) Asn breaks down to Asp and NH 4 + ___________ 3. When the following peptide is fully digested by trypsin, how many peptide fragments are expected? 4 | 3 | 2 | 1 Gly-His-Thr - Trp-Pro-Lys - Tyr-Ser-Phe-Arg Ala-Lys B) 4 three cuts, four fragments. Pro does not block hydrolysis when it is on the amino side of Lys. ___________ 4. What is the pH of the buffer formed by adding 0.080 mol of HCl to 0.100 mol tris base in a total volume of 1.00 L, given pKa = 8.10 for tris base? B) 7.50 after reaction with HCl, the solution contains 0.080 mol trisH + and leaves 0.020 mol tris pH = 8.1 + log (0.020 / 0.080) = 8.1 - 0.60 5. Identify the amino acid drawn at the right: C) Pro 6. Inhibition of an enzyme can be identified as competitive if kinetic measurements show that, when inhibitor is present: D) K M increases but V max is unchanged
Background image of page 2
7. In gel filtration chromatography, what property determines which protein is eluted first ?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/21/2011 for the course BIOOC 2580 taught by Professor Douger during the Fall '10 term at University of Guelph.

Page1 / 7

Fall 2002 Midterm Answers - Name _ Student number _...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online