3. MICROBIAL CONTROL CHEMICAL AGENTS.pptx - MICROBIAL...

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MICROBIAL CONTROL: CHEMICAL METHODS
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CHEMICAL CONTROL METHODS USUALLY ARE INTENDED FOR DISINFECTION AND ANTISEPSIS Chemical control methods generally do not achieve sterile conditions, although a few do. The principle applications of chemicals are disinfection and antisepsis. Several tests are available for evaluating the effectiveness of disinfectants and antiseptics. Phenol coefficient Disk diffusion Use dilution
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THE PHENOL COEFFICIENT TEST COMPARES THE EFFECTIVENESS OF THE AGENT TO PHENOL Phenol is the oldest chemical control agent, having been introduced into use by Joseph Lister in the 19 th century. The phenol coefficient test compares the killing efficiency of the agent to phenol. Identical amounts of Staphylococcus aureus or Salmonella typhi are added to each tube. Samples are withdrawn and the concentration at which all test bacteria are killed after 10 minutes but not after 5 minutes are determined.
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