5.3 StudyGuide.docx - BIO 264 | Module 5.3 Membrane Potentials Study Guide Module 5.3 Study Guide Module 5.3 Objectives Explain the principles behind

5.3 StudyGuide.docx - BIO 264 | Module 5.3 Membrane...

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BIO 264 | Module 5.3: Membrane Potentials Study Guide Module 5.3 Objectives: Explain the principles behind how an electrochemical gradient impacts the transfer of ions through a semi-permeable membrane. Describe how voltage-gated ion channels open and close in response to changes in voltage or ion movement in the membrane. Describe each phase of the action potential as it depolarizes and repolarizes. Describe the process of how action potentials propagate from cell to cell. Explain the role of the various protein carriers, protein channels, and ions involved in establishing the resting membrane potential, the graded potential, and the action potential 5.3 Introduction to Electrophysiology 5.3.1- Ions and Cell Membranes Be able to explain the principles behind how an electrochemical gradient impacts the transfer of ions through a semi-permeable membrane. Consider the following questions. o Define what Electrophysiology is and how a cell changes in electrical charge. It’s the study of electrical properties of cells. The cell membrane potential changes when charged particles move in and out of the cell . o What proteins and ions are important in establishing the resting membrane potential? Na+, K+, CL- and also negatively charged proteins inside of the cell . Explain how charged ions are separated in the cell. o Sodium/ potassium ATPase pumps use ATP to pump 3 Na+ out of the cell while pumping in 2 K+ in . What does ‘ potentia l’ mean in relation to the electrical properties of cells? It means having an electrical charge o What is the difference between resting membrane potential and action potential ? This separation occurs through the action of an ATP driven pump called the sodium/potassium ATPase pump Write in your own words what is meant by the term ‘electrochemical gradient’. Summarize the principles learned about ions diffusing across a Page 1 Module 5.3: Study Guide
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BIO 264 | Module 5.3: Membrane Potentials Study Guide semipermeable membrane from the two-chamber analogy given in the reading. There is a balance between concentration gradients and electrical gradients. The K+ can moves across the membrane and down the concentration gradients. Eventually the electrical forces grow strong enough to stop the K+ and creates a state where the inside of the membrane has a net negative charge and the outside will be positive . 5.3.2- Membrane Potentials Using all that you learn from reading 5.3.2 , explain the role of the various protein carriers, protein channels, and ions involved in establishing the resting membrane potential. Draw a diagram of the cell that illustrates the interactions involved in creating membrane potential in the cell. Include in your diagram the following: o The action of the main transporters in regulating the exchange of Na+ and K+ in and out of the cell: Sodium/Potassium ATPase pumps It created a chemical gradient for the Na+ and Na+. The
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