Chapter 20 Biotechnology notes

Chapter 20 Biotechnology notes - 1 Biotechnology a...

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1. Biotechnology a. Applications of DNA: i. Classic biotechnology – fermentation, cheese production, wine, yogurt, beer production, etc. ii. Recombinant DNA technology uses enzymes to splice and form new DNA molecules not normally found in nature. 1. Splicing genes from one organism into another (produces transgenic organism ). Trans means “across.” b. Making Recombinant DNA i. Restriction enzymes cut double-stranded DNA into fragments . These enzymes do not end in the suffix –ase. ii. Fragments base-pair at sticky ends. c. Restriction Enzymes i. Restriction enzymes cut double-stranded DNA. ii. Restriction enzymes recognize palindromic sequences in the DNA (also known as inverted repeats). 1. Bob, otto, kayak, dad, mom, Hannah are palindromic words. iii. Some (not all) restriction enzyme give rise to “ sticky ends .” These are ss DNA strands complimentary to other ss DNA strands (complementary base pairing). iv. Other restriction enzymes gives rise to blunt ends .
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