Lect7Lect8-Phages and DNAStructure

Lect7Lect8-Phages and DNAStructure - Lecture outline...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture outline Conjugation Transformation Bacteriophage characteristics Transduction Mapping in bacteriophages
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Transformation Introduction of extracellular DNA into a bacterium Can lead to incorporation in the chromosome Requires Entry of DNA into recipient (competent) cell Recombination of DNA with homologous sequences
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacteriophages Viruses that infect bacteria (narrow host range) Life cycle leads to inhibition of host synthesis and DNA degradation, while producing viral components The Plaque Assay is used to calculate number of phage Lysogeny results in incorporation of bacteriophage DNA prophage
Background image of page 4
Bacteriophage life cycle Adsorption/DNA extrusion Inhibition of host synthesis/Synthesis of viral DNA Degradation of host DNA/Phage proteins synthesis Assembly and host cell lysis/release
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Plaque assay
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Lecture outline Conjugation Transformation Bacteriophage characteristics Transduction Mapping in bacteriophages
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 43

Lect7Lect8-Phages and DNAStructure - Lecture outline...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online