Lect16-RecDNATech

Lect16-RecDNATech - Recombinant DNA Technology Outline DNA...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Recombinant DNA Technology
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Outline DNA cloning Restriction enzymes Vectors Cloning steps The Polymerase Chain Reaction DNA libraries Characterizing cloned sequences Restriction mapping Southern/Northern Blotting DNA sequencing
Background image of page 2
Outline DNA cloning Restriction enzymes Vectors Cloning steps The Polymerase Chain Reaction DNA libraries Characterizing cloned sequences Restriction mapping Southern/Northern Blotting DNA sequencing
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Making Recombinant DNA DNA is purified from cells/tissues DNA is cut with restriction enzymes at specific sequences DNA fragments cut with Restriction enzymes are joined to vectors Recombinant DNA molecule is transferred to host cell where it replicates During cell division, recombinant DNA molecule are passed to progeny
Background image of page 4
Restriction Enzymes Produced in bacteria Cut invading viral DNA Have a recognition sequence Cut both strands of DNA at sequence in a specific pattern Produce three types of ends
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 50

Lect16-RecDNATech - Recombinant DNA Technology Outline DNA...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online