Outline - Chapter 8

Outline - Chapter 8 - Chapter8(pages501531...

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Chapter 8 (pages 501-531) A stem cell story (http://eurostemcell.org/films/a-stem-cell-story/English) - 15 min movie 1. Isolating cells and grouping them in culture – why? - Tissues – heterogeneous cell populations - Cells attached to each other and to the extracellular matrix o Animal tissues  proteolytic enzymes (trypsin and collagenase) chelating agents (EDTA) o plant tissues pectinase - Separation of cells o Antibody (coupled to a fluorescent dye) binding to a specific cell types  FACS o Microdissection - Cell culture o Primary cultures – cells obtained directly from the tissues o Secondary cultures – recultured cells o Transformed cell lines  o Stem cells o Cloning – reproductive and therapeutic o Hybridomas – monoclonal antibodies 2. Protein purification - Cell fractionation o Centrifugation Velocity sedimentation – separation based on size and shape Equilibrium sedimentation – based on buoyant density
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Outline - Chapter 8 - Chapter8(pages501531...

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