02_lecture - Chapter2 TheStudyofMicrobial Structure:

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Click to edit Master subtitle style  Copyright © The   Chapter 2 The Study of Microbial  Structure:   Microscopy and Specimen  Preparation 1
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 Copyright © The  microorganisms range in size from the smallest,  viruses which are measured in nanometers (nm),  to the largest, which are about 200 micrometers  (μm). 2 Microscopy
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 Copyright © The  3 Table 2.1
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 Copyright © The  Light is refracted (bent) when passing  from one medium to another Refractive index a measure of how greatly a substance  slows the velocity of light Direction and magnitude of bending is  determined by the refractive indices of  4 Lenses and the Bending of 
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 Copyright © The  5 Figure 2.1
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 Copyright © The  Focus light rays at a specific place called the focal  point Distance between center of lens and focal point is  the focal length Strength of lens related to focal length short focal length  more magnification 6 Lenses
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 Copyright © The  7 Figure  2.2
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 Copyright © The  Many varieties bright-field microscope dark-field microscope phase-contrast microscope fluorescence microscope confocal microscope Are compound microscopes image formed by action of O 2 lenses 8 The Light Microscope
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 Copyright © The  Produces a dark image against a brighter  background Has several objective lenses parfocal microscopes remain in focus when  objectives are changed Total magnification  product of the magnifications of the ocular lenses  9 The Bright-Field Microscope
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 Copyright © The  10 Figure 2.3
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 Copyright © The  Ability of a lens to separate or distinguish small  objects that are close together 11 Microscope Resolution
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 Copyright © The  12 Figure  2.5 If air is replaced with immersion oil, many light rays  that did not enter the objective due to reflection and  refraction at the surfaces of the objective lens and  slide will now do so. This results in an increase in  resolution and numerical aperture.
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 Copyright © The  Image is formed by light reflected or refracted by  specimen Produces a bright image of the object against a dark  background Used to observe living, unstained preparations has been used to observe internal structures in  eukaryotic microorganisms has been used to identify bacteria such as  Treponema pallidum , the causative agent of  syphilis 13 The Dark-Field Microscope
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02_lecture - Chapter2 TheStudyofMicrobial Structure:

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