Presentation 12

Presentation 12 - Lecture 12! Lewins GENES X ! Chapter 5!...

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1 Lecture 12 Lewin’s GENES X Chapter 5 Genome Content Molecular Mapping Eukaryotic Genomes nonrepetitive and repetitive DNA How do we isolate a gene of interest? Genomes of organelles Polymorphism may be detected: at the phenotypic level when a sequence affects gene function at the restriction fragment level when it affects a restriction enzyme target site at the sequence level by direct analysis of DNA http://www.personalgenomes.org/
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2 GENOME CONTENT Genome : complete set of genes of an organism. chromosomal sequence DNA in organelles Transcriptome: complete set of RNAs in a cell, tissue, or organism. Proteome: complete set of proteins that is expressed by the entire genome. Protein Small nuclear RNAs Small nucleolar RNAs microRNAs short interfering RNAs regulate expression INTERACTOME The complete set of protein complexes/ protein–protein interactions present in a cell, tissue, or organism.
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3 Genomes Can Be Mapped at Several Levels of Resolution Linkage (genetic) maps are based on the frequency of recombination between genetic markers Restriction maps are based on the physical distances between markers. Molecular characterization of mutations can be used to reconcile linkage maps with physical restriction maps. Important for identifying disease genes
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4 Mapping Genomes Individual Genomes Show Extensive Variation Figure 5.1 * GAAATC CTTTAC
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5 …….A Molecular Pedigree Figure 5.2 * Mother Restriction site polymorphisms are inherited according to Mendelian rules
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6 Figure 5.3 * A restriction polymorphism can be used as a genetic marker to measure recombination distance from a phenotypic marker (such as eye color)
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7 Figure 5.4 * If a restriction marker is associated with a phenotypic characteristic, the restriction site must be located near the gene responsible for the phenotype
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8 Eukaryotic genomes: nonrepetitive and repetitive DNA sequences Selfsh DNA describes sequences that do not contribute to the genotype oF the organism but have selF-perpetuation within the genome as their sole Function. The kinetics oF DNA reassociation
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This note was uploaded on 09/25/2011 for the course GEN 409 taught by Professor Linda during the Spring '09 term at Iowa State.

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Presentation 12 - Lecture 12! Lewins GENES X ! Chapter 5!...

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