Chapter5 - Chapter 5: Gene Exploration 1. 2. 3. 4. Basic...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 5: Gene Exploration 1. Basic tools of gene exploration 2. Recombinant DNA technology 3. Whole genome sequencing 4. Genetic engineering Leading to: “Genomics”, recombinant drugs and organisms
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Recombinant DNA Technology Recombinant DNA technology, Genetic engineering, Gene cloning, and Molecular cloning . ..are all ways to say essentially the same thing. They mean: isolating desired DNA fragments joining them in new combinations and introducing the newly combined DNA into a living organism.
Background image of page 2
Molecular Cloning There are five steps to make a biotechnology product: 1. Select and identify a DNA molecule to serve as the carrier ( vector ) for the foreign DNA 2. Cleave the deoxyribonucleotide strands of the vector with a restriction endonuclease 3. Prepare and insert the foreign DNA fragment into the vector. This produces a recombinant or hybrid DNA molecule. 4. Introduce the hybrid DNA into a host organism (bacteria, plant, animal) where it can be replicated. This is called transformation . 5. Develop a method for identifying and screening host cells that have accepted and are replicating the hybrid DNA. …and finally purify the protein product if that is your goal
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Boyer, Cohen, and Berg Several major events: Isolation of E. coli suitable as host strain Development of plasmid DNA and bacteriophage DNA as cloning vectors Discovery of restriction endonucleases, ligase Basically you want to think of working with DNA before and after these techniques as writing before and after word processing. Think of the important functions of word processing, and there is an analogous molecular biology tool for DNA: Molecular Cloning Cutting copying rearranging pasting reading Writing
Background image of page 4
Herb Boyer Stanley Cohen
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Restriction enzymes (or “endonucleases”) found in prokaryotes Hamilton Smith Werner Arber E. co li Hae mophilus aegyptius Generally cleave palindromic sequence motifs
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EcoRV
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/25/2011 for the course BIOL 404 taught by Professor Dr.hargrove during the Spring '11 term at Iowa State.

Page1 / 39

Chapter5 - Chapter 5: Gene Exploration 1. 2. 3. 4. Basic...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online