Bchm3300_P1 - Biochemistry 3300 Problem Set 1 (1) Provide a...

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Biochemistry 3300 Problem Set 1 (1) Provide a reasonable systematic name for an enzyme that catalyzes the following reaction: fructose + ATP ―> fructose-1 phosphate + ADP (2) The IUBMB has a developed a set of rules for classifying enzymes based upon the type of reaction they catalyze. The classification scheme assigns an Enzyme Commission (EC) number to each enzyme. Answer the following questions regarding EC numbers and the class of reaction catalyzed: (a) What name is associated with class 4 enzymes and what type of reaction do they catalyze? (b) Oxidoreductases catalyze redox reactions. What class of enzymes are oxidoreductases? (c) What types of reactions are catalyzed by ligases? (3) There are many mechanisms that enzymes utilize to enhance reaction rates. Explain how the following mechanisms enhance reaction rates: (a) Covalent catalysis (b) Proximity effects (4) List the four principle characteristics of any metabolic pathway. (5) Identify two amino acids that commonly act as nucleophiles in enzyme catalyzed reactions. (6) What is the difference between an (1) isomerization reaction and (2) a rearrangement? (7) Enzyme catalyzed reactions that make or break C-C bonds typically involve carbanion intermediates. One means of stabilizing a carbanion intermediate involves the formation of a Schiff's base. Draw a strcuture of : (a) a Schiff's base carbanion (b) the resonance stabilized Schiff's base in the eneamine form (8) How can metabolic inhibitors be used to determine the sequence of reactions in a metabolic pathway? (9) Isotopes of C, N, S, P and H have been extensively used to investigate metabolic pathways. Why? (10) The standard transformed free energy change for the following bimolecular reaction: Glucose + ATP ----> Glucose-6-phosphate + ADP is -16.7 kJ/mol. Consequently, this reaction is spontaneous under standard conditions. Under what
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Bchm3300_P1 - Biochemistry 3300 Problem Set 1 (1) Provide a...

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