118K Column Chromatography

118K Column Chromatography - Michael Tam (mht347) 3/7/2011...

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Michael Tam (mht347) 3/7/2011 Column Chromatography Via the method of normal phase chromatography, a mixture of two compounds was separated. This was accomplished by capitalizing on the difference in polarity. The two resulting samples were then analyzed by comparing their melting point to the MSDS information of the two compounds. TLC was not used because we ran out of time. Column Chromatography uses gravity to pull the mixture (dissolved in an appropriate solvent) down the column. Packing material, usually silica or alumina separates the material based on polarity. The packing material is usually silica or alumina because they have uniform particle size and high specific area. This is important so that the bands are narrow, and that the bands are uniform. Column chromatography is different from TLC because instead of only microgram amounts of substance, grams of substance can be separated (although it takes a considerable amount of time more).
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This note was uploaded on 09/25/2011 for the course CH 118K taught by Professor Fjetland during the Spring '08 term at University of Texas at Austin.

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118K Column Chromatography - Michael Tam (mht347) 3/7/2011...

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