MBIO 3812 Additional Info Ex 3-5 & 3-6 Simple & Negative Stains

MBIO 3812 Additional Info Ex 3-5 & 3-6 Simple & Negative Stains

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
hromophore gives the stain its color) hemical link uxochrome charged portion of the stain) + POSITIVE STAIN (basic) coli EGATIVE STAIN (acidic) MBIO 3812 Exercises 3-5 Simple Stains and 3-6 Negative Stains If you were to put a bacterial sample on a slide and view it through a microscope you would not be able to see anything regardless of the magnification used. This is because the cytoplasm of a bacterial cell is transparent, which means that there is no contrast between the bacterial cell and the light passing up through the specimen. Therefore, the bacterial specimen must be treated in order to provide the necessary contrast and the easiest way to do this is by staining the sample. A stain is simply a solution to which a colored molecule known as a chromogen has been added. A chromogen consists of two components: (i) a chromophore , which gives the stain its color and (ii) an auxochrome , which possesses a charge. These 2 components are linked by a covalent bond.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern