MBIO 3812 Additional Info Ex 3-5 & 3-6 Simple & Negative Stains

MBIO 3812 Additional Info Ex 3-5 & 3-6 Simple & Negative Stains

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hromophore gives the stain its color) hemical link uxochrome charged portion of the stain) + + + + + + + + + + + + POSITIVE STAIN (basic) coli EGATIVE STAIN (acidic) MBIO 3812 Exercises 3-5 Simple Stains and 3-6 Negative Stains If you were to put a bacterial sample on a slide and view it through a microscope you would not be able to see anything regardless of the magnification used. This is because the cytoplasm of a bacterial cell is transparent, which means that there is no contrast between the bacterial cell and the light passing up through the specimen. Therefore, the bacterial specimen must be treated in order to provide the necessary contrast and the easiest way to do this is by staining the sample. A stain is simply a solution to which a colored molecule known as a chromogen has been added. A chromogen consists of two components: (i) a chromophore , which gives the stain its color and (ii) an auxochrome , which possesses a charge. These 2 components are linked by a covalent bond. It is the auxochrome portion of the chromogen, which actually dictates whether a stain
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MBIO 3812 Additional Info Ex 3-5 & 3-6 Simple & Negative Stains

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