MBIO 3812 EI report - MBIO 3812: Environmental Isolate (EI)...

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MBIO 2815 11 MBIO 3812: Environmental Isolate (EI) Report 75 points DUE DEC 8th Why are you doing this? learn good aseptic techniques learn to read, record and organize experimental data in a lab notebook learn/understand what is required to obtain a presumptive identification of an unknown bacterial isolate (your EI)
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MBIO 2815 22 MBIO 3812: Environmental Isolate (EI) Report EI assignment began on Wednesday (August 24th) or Thursday (August 25th) when you collected an environmental sample and inoculated the sample onto a TSA plate (why TSA?) Today, examine your plate. Do you have many colonies? Do you have colonies that show different morphologies? (what does this mean?)
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MBIO 2815 33 MBIO 3812: Environmental Isolate (EI) Report From today (Aug 29th or 30th) to Sept 14th or 15th GOAL: to obtain pure cultures of 3 distinct bacterial species from your environmental sample To achieve this goal will need to sub-culture 3 different bacterial species on TSA plates until you obtain a plate for each that has ONLY 1 colony type (assumed to be pure)
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MBIO 3812 EI report - MBIO 3812: Environmental Isolate (EI)...

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