MBIO 3812 Ex 1-2 Media Making

MBIO 3812 Ex 1-2 Media Making - MBIO 3812 Exercise 1-2...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
9/26/11 11 Exercise 1-2 Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA) Preparation MBIO 3812 In order to cultivate and maintain bacterial cultures nutrients such as carbon and nitrogen sources, amino acids, carbohydrates and lipids must be provided These nutrients are supplied to bacteria in MEDIA that can be either a liquid ( BROTH ) or a solid ( AGAR ) form Since bacteria have diverse nutritional requirements many different types of media have been developed Depending upon the ingredients it is composed of media is considered either UNDEFINED or DEFINED
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9/26/11 22 MBIO 3812 UNDEFINED MEDIA The exact ingredients and their amounts are not known Example: beef extract a component in many different types of media is the concentrated broth that comes from cooking beef Commercially available and relatively easy to prepare DEFINED MEDIA All of the ingredients and their amounts are known Since there are many different ingredients required in the preparation of this type of media it is more complicated and time consuming to prepare Exercise 1-2 Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA) Preparation
Background image of page 2
9/26/11 33 UNDEFINED MEDIA 8 grams Tryptic Soy broth + 200 ml water DEFINED MEDIA MBIO 3812 Exercise 1-2 Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA) Preparation
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9/26/11 44 In microbiological media, AGAR is the component that is used as a solidifier Agar originates from seaweed Agar is a better solidifier than gelatin because it is firmer/stronger
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 16

MBIO 3812 Ex 1-2 Media Making - MBIO 3812 Exercise 1-2...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online