MBIO 3812 Ex 1-3 Aseptic transfers & Inoculations

MBIO 3812 Ex 1-3 Aseptic transfers & Inoculations -...

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9/26/11 11 MBIO 3812 Exercise 1-3 Common Aseptic Transfers and Inoculation Methods Purpose of this exercise: learning how to transfer bacteria using sterile techniques What is ESSENTIAL when transferring bacteria to a sterile broth, slant or agar plate is to maintain sterility by using aseptic techniques ( i.e. , preventing the media from becoming contamination) Transferring bacteria is required for maintaining a bacterial culture as well as using a bacterial culture in any type of morphological, physiological or biochemical test
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9/26/11 22 Why are aseptic techniques so important? Contamination of a culture with additional bacterial species can confound results used in the identification of bacterium (this will be extremely important while working to identify your environmental isolate!) MBIO 3812 Exercise 1-3 Common Aseptic Transfers and Inoculation Methods The development of good aseptic techniques is one of several fundamental microbiological cornerstones
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