MBIO 3812 Ex 1-5 & 6-1 Spread Plates Viable Counts

MBIO 3812 Ex 1-5 & 6-1 Spread Plates Viable Counts -...

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11 MBIO 3812 Ex 1-5 Spread Plate Method of Isolation Ex 6-1 Standard Plate Count (Viable Counts) Purpose of Exercise: To learn how to determine/quantify the number of bacterial COLONIES in a bacterial culture This is an especially important consideration for food and water safety Is the level of bacteria in our drinking water acceptable by local, state or federal standards? Is the lettuce in the processing plant contaminated and if so what is the level?
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22 2 techniques must be mastered in order to determine how many BACTERIAL COLONIES are in a culture, water sample, food sample etc… 1. Making dilutions 1. Making spread plates MBIO 3812 Ex 1-5 Spread Plate Method of Isolation Ex 6-1 Standard Plate Count (Viable Counts)
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33 1. Making dilutions Supplemental material on D2L MBIO 3812 Ex 1-5 Spread Plate Method of Isolation Ex 6-1 Standard Plate Count (Viable Counts)
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44 2. Making spread plates requires several steps Step 1 label each plate according to the tube the sample will
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Ex 1-5 & 6-1 Spread Plates Viable Counts -...

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