MBIO 3812 Ex 2-1 Ubiquity

MBIO 3812 Ex 2-1 Ubiquity - Step #1 for the environmental...

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9/26/11 11 MBIO 3812 Exercise 2-1 Ubiquity of Microorganisms Ubiquity = existing or being everywhere Microorganisms are all around us AIR SOIL WATER ANIMALS HUMANS PLANTS
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9/26/11 22 MBIO 3812 Exercise 2-1 Ubiquity of Microorganisms What types of microorganisms are out there? Free-living microorganisms These microorganisms aren’t associated with any specific plant, or animal Non-pathogenic (not disease causing) Pathogenic microorganisms Disease causing Opportunistic pathogens Normally inhabit our bodies without causing disease These microorganisms can cause disease if introduced into a new area of the body or if the immune system is suppressed ( i.e ., if the conditions are right the microbes can seize the opportunity and cause disease)
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9/26/11 33 MBIO 3812 Exercise 2-1 Ubiquity of Microorganisms The importance of this exercise In this exercise you will collect a sample from outside the building, which will be serve as the source of your EI
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Unformatted text preview: Step #1 for the environmental isolate (EI) report 9/26/11 44 MBIO 3812 Exercise 2-1 Ubiquity of Microorganisms Why would you need to streak environmental samples differently than clinical samples? Unlike clinical samples, the amount of bacteria in an environmental sample is likely to be much lower If there is a low density of bacteria to begin with, quadrant streaking for isolation would not be effective since the bacteria would likely all appear in the 1st quadrant and not be well separated Quadrant streaking for isolation is difficult away from a lab setting since there is generally not a flame source available. 9/26/11 55 MBIO 3812 Exercise 2-1 Ubiquity of Microorganisms Using a swab a zigzag pattern is made down the plate The environmental streak The plate that contains your environmental sample should be incubated at 37C for ~48 hr prior to being examined...
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MBIO 3812 Ex 2-1 Ubiquity - Step #1 for the environmental...

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