MBIO 3812 Ex 3-5 & 3-6 Simple & Negative Stains

MBIO 3812 Ex 3-5 & 3-6 Simple & Negative...

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11 MBIO 3812 Exercises 3-5 & 3-6 Simple and Negative Stains Why do bacteria need to be stained in order to be seen? The cytoplasm of bacterial cells is TRANSPARENT Stains/dyes provide CONTRAST , which allows the bacteria to be visualized Without staining bacteria on a slide would appear as such when view under the microscope.
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22 MBIO 3812 What is a stain? a solution in which a dye or CHROMOGEN (chromo = color How does a stain work? a chromogen consists of 2 components Chromophore (gives the stain its color) chemical link Auxochrome (the charged portion of the stain) Exercises 3-5 & 3-6 Simple and Negative Stains
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33 MBIO 3812 How does a stain work? it is the CHARGE carried by the auxochrome that determines whether or not a stain “sticks" to a bacterium or is repelled away from a bacterium Stains are positive or negative based on the charge carried by the auxochrome Exercises 3-5 & 3-6 Simple and Negative Stains
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44 MBIO 3812 E. coli POSITIVE STAIN (basic) Bacteria have a negative charge on their cell surface auxochrome has a positive charge opposites attract auxochrome binds to bacterial cell surface chromophore attached to auxochrome bacteria is stained & background is white Exercises 3-5 & 3-6 Simple and Negative Stains + + + + + + + + + +
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55 MBIO 3812 E. coli NEGATIVE STAIN (acidic) Bacteria have a negative charge on their cell surface Exercises 3-5 & 3-6 Simple and Negative Stains
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Ex 3-5 & 3-6 Simple & Negative...

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