MBIO 3812 Ex 3-8 Acid fast stain

MBIO 3812 Ex 3-8 Acid fast stain - MBIO 3812 Ex 3-8...

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11 MBIO 3812 Ex 3-8 Acid-Fast Stains The Gram stain is the most common stain used in microbiology and the results provide key information for the identification of a bacterium. The Gram stain does NOT work on certain species of bacteria specially bacteria in the genus Nocardia and Mycobacterium BUT…… WHY?
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22 MBIO 3812 Ex 3-8 Acid-Fast Stains Nocardia and Mycobacterium species have. .. A UNIQUE CELL WALL STRUCTURE Gram positive Gram negative
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33 MBIO 3812 Ex 3-8 Acid-Fast Stains Nocardia and Mycobacterium species have. .. A UNIQUE CELL WALL STRUCTURE Mycobacterium cell wall Inner membrane Peptidoglycan Mycolic acid ( waxy lipid )
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44 MBIO 3812 Ex 3-8 Acid-Fast Stains A Gram stain will NOT work because… 1. Dyes have difficulty penetrating the bacterium due to its waxy cell wall. 2. Dyes that do enter the bacterium are difficult to remove because of the bacterium's waxy cell wall. Doing a Gram stain will give you inaccurate results! inner membrane peptidoglycan mycolic acid
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55 MBIO 3812 Ex 3-8 Acid-Fast Stains Why is it important to determine if a bacterium has a waxy cell wall? Mycobacterium tuberculosis , which causes tuberculosis (TB), has a waxy cell wall and is highly communicable. 1/3 of the world’s population is infected with TB Almost 2 million TB-related deaths each year TB leading killer of HIV infected individuals
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MBIO 3812 Ex 3-8 Acid fast stain - MBIO 3812 Ex 3-8...

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