MBIO 3812 Ex 3-13 Flagella Stain

MBIO 3812 Ex 3-13 Flagella Stain - = 1 flagella at 1 pole...

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11 MBIO 3812 Ex 3-13 Flagella Stain flagella provides a bacterium with the means to move towards more favorable conditions (e.g. an area with more nutrients) NOT ALL bacteria have flagella so whether a bacterium is motile or not can help in its identification flagella may also contribute to the virulence of a bacterium since it can allow the bacterium to move to its preferred anatomical site to start the disease process
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22 MBIO 3812 Ex 3-13 Flagella Stain How to determine whether a bacterium is motile 1. A hanging drop slide can be prepared from a broth culture and directly examined to determine if the bacteria are moving 2. By using semi-solid motility agar (see Ex 5-28 for more details) But neither of these techniques provides specific information about the flagella itself
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33 MBIO 3812 Ex 3-13 Flagella Stain Types of flagella flagella can be in different arrangements , which provides additional information used in the identification of the bacterium
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44 MBIO 3812 Ex 3-13 Flagella Stain MONOTRICHOUS
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Unformatted text preview: = 1 flagella at 1 pole poles 55 MBIO 3812 Ex 3-13 Flagella Stain LOPHOTRICHOUS = several flagella at 1 pole 66 MBIO 3812 Ex 3-13 Flagella Stain AMPHITRICHOUS = 1 flagella at each pole 77 MBIO 3812 Ex 3-13 Flagella Stain PERITRICHOUS = flagella all over 88 MBIO 3812 Ex 3-13 Flagella Stain bacterial flagella are EXTREMELY THIN and therefore cannot be seen in a light microscope even when oil immersion is used need to use a MORDANT that will stick/bind to the flagella and thicken it enough that it can be seen under oil immersion Visualization of flagella 99 MBIO 3812 Ex 3-13 Flagella Stain Problems associated with the flagella stain flagella are thin but also VERY DELICATE and can be easily broken off from the bacterium during the staining procedure the mordant used is NON-SPECIFIC meaning that it will bind/stick to the flagella as well as any dust or dirt on the slide (e.g., the oil on your finger tips)...
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MBIO 3812 Ex 3-13 Flagella Stain - = 1 flagella at 1 pole...

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