MBIO 3812 Ex 3-13 Flagella Stain

MBIO 3812 Ex 3-13 Flagella Stain - = 1 flagella at 1 pole...

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11 MBIO 3812 Ex 3-13 Flagella Stain flagella provides a bacterium with the means to move towards more favorable conditions (e.g. an area with more nutrients) NOT ALL bacteria have flagella so whether a bacterium is motile or not can help in its identification flagella may also contribute to the virulence of a bacterium since it can allow the bacterium to move to its preferred anatomical site to start the disease process
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22 MBIO 3812 Ex 3-13 Flagella Stain How to determine whether a bacterium is motile 1. A hanging drop slide can be prepared from a broth culture and directly examined to determine if the bacteria are moving 2. By using semi-solid motility agar (see Ex 5-28 for more details) But neither of these techniques provides specific information about the flagella itself
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33 MBIO 3812 Ex 3-13 Flagella Stain Types of flagella flagella can be in different arrangements , which provides additional information used in the identification of the bacterium
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44 MBIO 3812 Ex 3-13 Flagella Stain MONOTRICHOUS
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Unformatted text preview: = 1 flagella at 1 pole poles 55 MBIO 3812 Ex 3-13 Flagella Stain LOPHOTRICHOUS = several flagella at 1 pole 66 MBIO 3812 Ex 3-13 Flagella Stain AMPHITRICHOUS = 1 flagella at each pole 77 MBIO 3812 Ex 3-13 Flagella Stain PERITRICHOUS = flagella all over 88 MBIO 3812 Ex 3-13 Flagella Stain • bacterial flagella are EXTREMELY THIN and therefore cannot be seen in a light microscope even when oil immersion is used • need to use a MORDANT that will stick/bind to the flagella and “thicken” it enough that it can be seen under oil immersion Visualization of flagella 99 MBIO 3812 Ex 3-13 Flagella Stain Problems associated with the flagella stain • flagella are thin but also VERY DELICATE and can be easily “broken off” from the bacterium during the staining procedure • the mordant used is NON-SPECIFIC meaning that it will bind/stick to the flagella as well as any dust or dirt on the slide (e.g., the oil on your finger tips)...
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Ex 3-13 Flagella Stain - = 1 flagella at 1 pole...

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