MBIO 3812 Ex 5-4 MR-VP Test

MBIO 3812 Ex 5-4 MR-VP Test - 11 MBIO 3812 Ex 5-4 Methyl...

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Unformatted text preview: 11 MBIO 3812 Ex 5-4 Methyl Red & Voges-Proskauer Tests There are several different fermentation pathways producing its own characteristic set of unique end products Not all bacterial species use the same fermentation pathways, therefore knowing what types of fermentation a bacterium is capable of performing can be used in the identification of that organism 22 MBIO 3812 Ex 5-4 Methyl Red & Voges-Proskauer Tests The Methyl Red (MR) & Voges-Proskauer (VP) tests are used to determine whether a bacterium can perform mixed acid fermentation or 2,3-butanediol fermentation respectively MR test VP test MIXED ACID fermentation GLUCOSE PYRUVAT E PHOSPHOENOLPYRUVAT E (PEP) LACTIC ACID ACETIC ACID FORMIC ACID CO2 H2 gas Ethanol SUCCINIC ACID GLYCOLYSI S 33 MBIO 3812 Ex 5-4 Methyl Red & Voges-Proskauer Tests Involves the conversion of pyruvate, through a series of enzymatic reactions, to several stable acids as well as CO2, H2 gas and ethanol MR-VP Broth (same broth for both tests) Only 3 media components: 1. Peptone , which acts as a source of proteins 2. Glucose 3. Phosphate buffer , which maintains the media at a neutral pH phosphate buffer only allows the media to remain neutral when LOW amounts of acid are present, LARGE amounts of acidic compounds will cause the pH to drop the media does NOT contain a pH indicator 44 MBIO 3812 Ex 5-4 Methyl Red & Voges-Proskauer Tests...
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MBIO 3812 Ex 5-4 MR-VP Test - 11 MBIO 3812 Ex 5-4 Methyl...

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