MBIO 3812 Ex 5-7 Nitrate Reduction Test

MBIO 3812 Ex 5-7 Nitrate Reduction Test - MBIO 3812 Ex 5-7...

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11 MBIO 3812 Ex 5-7 Nitrate Reduction Test CATABOLIC PATHWAYS GLUCOSE enters the cell AEROBIC RESPIRATION ANAEROBIC RESPIRATION FERMENTATION Anaerobic respiration does NOT use O2 as a final electron acceptor but instead uses NONOXYGEN electron acceptors such as NITRATE (NO3-)
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if nitrate (NO3-) is used as the final electron acceptor than the bacteria can perform either nitrate respiration (i.e., DENITRIFICATION ) to produce nitrogen gas or AMMONIFICATION to produce ammonia MBIO 3812 Ex 5-7 Nitrate Reduction Test Glycolysis Krebs cycle ETC NO3 e-
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33 MBIO 3812 Ex 5-7 Nitrate Reduction Test Denitrification = a multi-step process performed by a group of enzymes that leads to the production of nitrogen gas from NO3 NO3 nitrate NO2 nitrite NO nitric oxide N2O nitrous oxide N2 nitrogen gas
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44 MBIO 3812 Ex 5-7 Nitrate Reduction Test Ammonification = a multi-step process performed by a group of enzymes that leads to the production of ammonia from NO3 NO3 nitrate NO2 nitrite NH4 ammonia
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55 MBIO 3812 Ex 5-7 Nitrate Reduction Test NO3 nitrate NO2 nitrite nitrate reductase The 1st step in either denitrification OR ammonification is the reduction of NITRATE (NO3) to NITRITE (NO2) by the enzyme NITRATE REDUCTASE
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Purpose of the nitrate reduction test: to determine if an organism can use nitrate for either denitrification or ammonification MBIO 3812 Ex 5-7 Nitrate Reduction Test NO2 nitrite NO nitric oxide N2O nitrous oxide N2 nitrogen gas NO3 nitrate NH4 ammonia
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Nitrate Broth Key components: 1. POTASSIUM NITRATE ( KNO3 ) the nitrate source 2. A Durham tube to monitor gas production MBIO 3812 Ex 5-7 Nitrate Reduction Test Nitrate broth does NOT contain any color indicators so prior to inoculation the broth is clear
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88 MBIO 3812 Ex 5-7 Nitrate Reduction Test Possible results from the nitrate reduction test: After overnight growth gas production
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Ex 5-7 Nitrate Reduction Test - MBIO 3812 Ex 5-7...

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