MBIO 3812 Ex 5-10 Decarboxylation Tests

MBIO 3812 Ex 5-10 Decarboxylation Tests - MBIO 3812 Ex 5-10...

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11 MBIO 3812 Ex 5-10 Decarboxylation Tests In protein catabolism proteolytic enzymes (proteases) are used to break down proteins into peptides and peptides down to their basic amino acid components Protein Peptides Amino acids
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THE BASIC STRUCTURE OF AN AMINO ACID Variable (R) group (different for each amino acid) CARBOXYL GROUP AMINE GROUP MBIO 3812 Ex 5-10 Decarboxylation Tests Specific enzymes can break down amino acids 22
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CARBOXYL GROUP MBIO 3812 Ex 5-10 Decarboxylation Tests 33 Decarboxylation = removal of the carboxyl group of an amino acid Decarboxylase = enzyme that catalyzes the removal of an amino acid’s carboxyl group
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MBIO 3812 Ex 5-10 Decarboxylation Tests 44 Decarboxylase amino acid amine CO2 pyridoxyl phosphate coenzyme required for decarboxylase activity
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SPECIFIC decarboxylases act on specific amino acids 55 MBIO 3812 Ex 5-10 Decarboxylation Tests Examples: Ornithine decarboxylase ONLY cleaves the carboxyl group from ornithine Arginine decarboxylase ONLY cleaves the carboxyl group from arginine Lysine decarboxylase ONLY cleaves the carboxyl group from lysine
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66 MBIO 3812 Ex 5-10 Decarboxylation Tests End products of decarboxylation Ornithine Lysine Arginine Polyamine Polyamine Polyamine ornithine decarboxylase arginine decarboxylase lysine decarboxylase Polyamine = organic compound with 2 or more primary amino (NH2) groups
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77 MBIO 3812 Ex 5-10 Decarboxylation Tests Importance of Polyamines: role in the regulation of gene transcription and translation role in biofilm formation associated with bacterial iron scavenging molecules involved in the bacterial response to low pH
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Ex 5-10 Decarboxylation Tests - MBIO 3812 Ex 5-10...

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