MBIO 3812 Ex 5-13 Urea Hydrolysis

MBIO 3812 Ex 5-13 Urea Hydrolysis - MBIO 3812 Ex 5-13 Urea...

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11 MBIO 3812 Ex 5-13 Urea Hydrolysis the product of the decarboxylation (removal of the carboxyl group) of certain amino acids Urea: an enzyme present in certain bacteria that can hydrolyze (break down) urea into AMMONIA (NH3) and CO2 Urease: UREASE Alkaline compound Produces low pH
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The presence of urease in enteric bacteria: several enteric bacteria that reside in the gut possess urease conditions in several parts of the gut can be acidic with the most extreme acidic environment (pH<2) in the stomach for a bacterium to survive in acidic conditions requires that the organism maintains a neutral internal pH urease breaks down urea into ammonia a strong alkaline compound because it is a basic compound ammonia can neutralize the acidic conditions within the bacterium MBIO 3812 Ex 5-13 Urea Hydrolysis 22
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Urea Broth: 1. A trace amount of yeast extract (the only nutrient source in the broth 2. Urea (the substrate for the enzyme) 3. Potassium phosphate buffer (strong enough to
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MBIO 3812 Ex 5-13 Urea Hydrolysis - MBIO 3812 Ex 5-13 Urea...

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