MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test

MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test - after...

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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test collagen protein found mostly in fibrous tissues gelatin product from the hydrolysis ( i.e. , break down) of collagen 11
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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 22 n Gelatin: large protein polymer made up of numerous peptide subunits abundant source of amino acids
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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 33 n Problem: gelatin is too large a molecule to be transported into the bacterial cell for a bacterium to catabolize gelatin requires an enzyme that can break down the protein into its amino acid subunits
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Gelatinase: MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 44 proteolytic enzyme that breaks down gelatin into its individual amino acid components by cleaving peptide bonds SECRETED from the bacteria
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Media used in the gelatin hydrolysis test: Nutrient Gelatin simple media that contains GELATIN ( i.e. , substrate for the enzyme) gelatin is a solidifying agent control media is firm and solid in consistency prior to inoculation MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 55
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Possible results form the Gelatin Hydrolysis Test:
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Unformatted text preview: after inoculation with the test bacteria and overnight growth media remains SOLID since media still solid gelatin , which is the solidifying agent, must be present gelatin NOT broken down MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 66 Organism is GELATINASE NEGATIVE Possible results form the Gelatin Hydrolysis Test: after inoculation with the test bacteria and overnight growth media BECOMES LIQUIFIED gelatin would solidify the media so it is nO longer present gelatin absent because it was broken down to break down the gelatin gelatinase must have been secreted MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 77 Organism is GELATINASE POSITIVE Gelatinase negative ( media is firm and does not move when the tube is slanted) Gelatinase positive (media is a liquid that runs along the walls of the tube if slanted) MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 88 Possible results form the Gelatin Hydrolysis Test:...
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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test - after...

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