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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test

MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test - • after...

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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test collagen protein found mostly in fibrous tissues gelatin product from the hydrolysis ( i.e. , break down) of collagen 11
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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 22 n Gelatin: large protein polymer made up of numerous peptide subunits abundant source of amino acids
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MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 33 n Problem: gelatin is too large a molecule to be transported into the bacterial cell for a bacterium to catabolize gelatin requires an enzyme that can break down the protein into its amino acid subunits
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Gelatinase: MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 44 proteolytic enzyme that breaks down gelatin into its individual amino acid components by cleaving peptide bonds SECRETED from the bacteria
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Media used in the gelatin hydrolysis test: Nutrient Gelatin simple media that contains GELATIN ( i.e. , substrate for the enzyme) gelatin is a solidifying agent control media is firm and solid in consistency prior to inoculation MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 55
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Possible results form the Gelatin Hydrolysis Test:
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Unformatted text preview: • after inoculation with the test bacteria and overnight growth media remains SOLID • since media still solid gelatin , which is the solidifying agent, must be present • gelatin NOT broken down MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 66 Organism is GELATINASE NEGATIVE Possible results form the Gelatin Hydrolysis Test: • after inoculation with the test bacteria and overnight growth media BECOMES LIQUIFIED • gelatin would solidify the media so it is nO longer present • gelatin absent because it was broken down • to break down the gelatin gelatinase must have been secreted MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 77 Organism is GELATINASE POSITIVE Gelatinase negative ( media is firm and does not move when the tube is slanted) Gelatinase positive (media is a liquid that runs along the walls of the tube if slanted) MBIO 3812 Ex 5-15 Gelatin Hydrolysis Test 88 Possible results form the Gelatin Hydrolysis Test:...
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