MBIO 3812 Ex 5-28 Motility Test

MBIO 3812 Ex 5-28 Motility Test - STRAIGHT out Motility can...

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11 MBIO 3812 Ex 5-28 Motility Test flagella staining can provide useful information about a bacterium’s flagella arrangement; however, the staining procedure is not easy to master and the overall success rate in being able to clearly visualize flagella is low for this reason bacteria are usually examined for motility rather than the presence of flagella FLAGELLA = MOTILITY The easiest way to determine if a bacterium is motile is with motility media
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22 MBIO 3812 Ex 5-28 Motility Test Motility media: A semi-solid media which has just enough agar added to produce a media with the consistency of “ sloppy Jello ” (~ 4X less agar than the media used to make agar plates) This media is thick enough to allow motile bacteria to move through it but is too thick for non-motile bacteria to disperse even as they replicate
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33 MBIO 3812 Ex 5-28 Motility Test Motility Test Procedure Inoculating needle that contains bacteria stabbed ONCE STRAIGHT into the middle of the motility media (¾ of the way down) and then pulled
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Unformatted text preview: STRAIGHT out Motility can be seen as diffuse growth radiating from the stab line To make a motility test easy to read TETRAZOLIUM SALT (TTC) can be added to the media 44 MBIO 3812 Ex 5-28 Motility Test Tetrazolium salt (TTC) reduction HCl + TTC When oxidized colorless & soluble Formazan (reduced form of TTC) Red & insoluble TTC is an electron acceptor that is used to detect cellular respiration when TTC is reduced by metabolically active bacteria it forms a red, insoluble compound in motility media TTC indicates the location of metabolically active /growing bacteria 55 MBIO 3812 Ex 5-28 Motility Test negative non-motile growth only along the stab line positive motile growth spread out from the stab line ( diffuse red color ) 66 MBIO 3812 Ex 5-28 Motility Test negative non-motile positive motile ( diffuse red color ) Must be careful not to jiggle the needle when stabbing the media as this may give the appearance of motility (false positive)...
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MBIO 3812 Ex 5-28 Motility Test - STRAIGHT out Motility can...

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