MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer

MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer - MBIO 3812 Ex...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
9/26/11 11 1 MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer There are a multitude of animal viruses that can infect humans and cause disease. Work with animal viruses cannot be done in this lab for several reasons: Obvious safety concerns
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9/26/11 22 2 MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer proper facilities and equipment not available A biosafety cabinet Work with animal viruses cannot be done in this lab for several reasons: working with viruses requires eukaryotic cells for virus replication working with viruses & eukaryotic cells requires a high level of sterility work must be done in biosafety cabinets that have a filtered air supply
Background image of page 2
9/26/11 33 BACTERIOPHAGE = viruses that only infect bacteria MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer Cannot work with animal viruses in the lab but there is another type of virus that can be used in the lab. ..
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9/26/11 44 Bacteriophage Animal Virus requires a host ( bacteria ) in which to replicate requires a host ( eukaryotic cell ) in which to replicate does not have enzymes, ribosomes etc… it uses the cellular machinery of its host does not have enzymes, ribosomes etc… it uses the cellular machinery of its host contains DNA contains RNA or DNA but never both complex structure simple structure MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer
Background image of page 4
9/26/11 55 A bacteriophage (T4) An animal virus (HIV) Capsid or head with DNA inside Tail fibers Sheath Bacteriophage are more complex in structure than most animal viruses MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9/26/11 66 Bacteriophage Animal Virus complex structure simple structure lyse bacteria lyse eukaryotic cells MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer
Background image of page 6
9/26/11 77 MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer Attachment Penetration Synthesis Assembly Release
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

Page1 / 20

MBIO 3812 Ex 6-5 Plaque Assay of Virus Titer - MBIO 3812 Ex...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online