MBIO 3812 Lab Notebook Example

MBIO 3812 Lab Notebook Example - Lab Notebook Example TABLE...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Lab Notebook Example TABLE OF CONTENTS Exercise/Procedure Date Performed Page 8/25/10 1 Ex 3-7 Gram Stain 9/13/10 15
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Lab Notebook Example Page 1 Ex 1-2 Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA) Preparation August 23, 2010 Purpose: To learn how to prepare microbiological media Methods: The procedure outlined in Ex 1-2 “Nutrient Broth and Nutrient Agar rd edition, Leboffe and Pierce was performed with the following modifications. Tryptic soy broth and agar were used in place of nutrient broth and agar. Tryptic soy broth and agar are undefined media composed of tryptone, soytone, sodium chloride and dipotassium phosphate rd edition, Appendix H, p 470). The TSA and TSB media was prepared as per the Media Recipe Supplemental Material posted on D2L. Briefly, for the TSA media 6 grams of tryptic soy agar was added to 150 ml of water in a 250 ml flask and then the media sterilized for 20 minutes at 121 ° C and 15 pounds pressure in the autoclave. Following autoclaving the media was cooled to ~50°C and then used to pour plates. Plates were allowed to solidify for ~20 minutes and once
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

Page1 / 3

MBIO 3812 Lab Notebook Example - Lab Notebook Example TABLE...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online