MBIO 3812 PHB Inclusion Body Stain

MBIO 3812 PHB Inclusion Body Stain - MBIO 3812 Supplemental...

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11 MBIO 3812 Supplemental Exercise: Poly β-hydroxybutyrate (PHB) Staining during a microbe's life time it will likely encounter many changes in the availability of nutrients in the environment to overcome this constant flux in nutrient concentrations some bacteria have the capacity to produce INCLUSION BODIES or GRANULES when nutrients are available in high concentrations some bacteria can accumulate and store these nutrients in inclusions within the cytoplasm when nutrients in the environment become scarce the macromolecules within these inclusions can be used by the bacteria to sustain its growth ( reserve deposits )
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22 MBIO 3812 inclusions in a bacterial cell are generally large enough to be seen under oil immersion the inclusion can vary in number in a bacterium if a bacterium is in a nutrient rich environment it is likely to contain more or larger inclusions in depleted nutrient environments there are more likely to be fewer or smaller inclusions since the bacterium may have utilized some of the stored macromolecules Supplemental Exercise: Poly β-hydroxybutyrate (PHB) Staining
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33 MBIO 3812 Examples of inclusions in bacteria Supplemental Exercise: Poly β-hydroxybutyrate (PHB) Staining
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44 MBIO 3812 Examples of inclusions in bacteria Supplemental Exercise: Poly β-hydroxybutyrate (PHB) Staining
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MBIO 3812 PHB Inclusion Body Stain - MBIO 3812 Supplemental...

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